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Treatment of Autoimmune Disease by Adoptive Cellular Gene Therapy
Author(s) -
TARNER INGO H.,
SLAVIN ANTHONY J.,
McBRIDE JACQUELINE,
LEVICNIK ALENKA,
SMITH RICHARD,
NOLAN GARRY P.,
CONTAG CHRISTOPHER H.,
FATHMAN C. GARRISON
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1254.067
Subject(s) - immunology , immune system , adoptive cell transfer , genetic enhancement , arthritis , cell therapy , nod , autoimmune disease , nod mice , antigen , t cell , medicine , biology , autoimmunity , cell , antibody , in vivo , gene , biochemistry , genetics , microbiology and biotechnology
A bstract : Autoimmune disorders represent inappropriate immune responses directed at self‐tissue. Antigen‐specific CD4+ T cells and antigen‐presenting dendritic cells (DCs) are important mediators in the pathogenesis of auto‐immune disease and thus are ideal candidates for adoptive cellular gene therapy, an ex vivo approach to therapeutic gene transfer. Using retrovirally transduced cells and luciferase bioluminescence, we have demonstrated that primary T cells, T cell hybridomas, and DCs rapidly and preferentially home to the sites of inflammation in animal models of multiple sclerosis, arthritis, and diabetes. These cells, transduced with retroviral vectors to drive expression of various “regulatory proteins” such as IL‐4, IL‐10, IL‐12p40, and anti‐TNF scFv, deliver these immunoregulatory proteins to the inflamed lesions, providing therapy for experimental autoimmune encephalitis (EAE), collagen‐induced arthritis (CIA), and nonobese diabetic mice (NOD).