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IL‐10 expression profiling in human monocytes
Author(s) -
Williams Lynn,
Jarai Gabor,
Smith Alexandra,
Finan Peter
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.72.4.800
Subject(s) - biology , cytokine , socs5 , gene , proinflammatory cytokine , socs6 , immunology , interleukin 10 , interleukin 19 , signal transduction , regulation of gene expression , gene expression , interleukin , gene expression profiling , microbiology and biotechnology , socs3 , inflammation , genetics , suppressor , interleukin 5
Interleukin‐10 (IL‐10) is a potent anti‐inflammatory cytokine with numerous immunomodulatory effects, including the inhibition of proinflammatory cytokine production. The mechanisms by which IL‐10 exerts these effects still remain largely unknown. As there is evidence that suggests IL‐10‐mediated cytokine suppression requires the induction of an intermediate gene, we have used gene‐chip technology to identify IL‐10‐inducible genes in human monocytes. We have been able to identify a total of 19 genes that are up‐regulated in response to IL‐10. Three of these genes had been identified previously: IL‐1ra, suppressors of cytokine signaling‐3, and CD163; however, the other 16 represent newly identified IL‐10‐responsive genes. Further analysis of the regulation of eight of these genes showed a remarkable specificity to regulation by lipopolysaccharides (LPS) and IL‐10, but not by other anti‐inflammatory mediators such as IL‐4 and transforming growth factor‐β, suggesting that two diverse stimuli such as IL‐10 and LPS may engage common signaling mechanisms.