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Platelet factor 4 induces human natural killer cells to synthesize and release interleukin‐8
Author(s) -
Martí Francesc,
Bertran Esther,
Llucià Montserrat,
Villén Esther,
Peiró Matilde,
Garcia Joan,
Rueda Fèlix
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.72.3.590
Subject(s) - biology , wortmannin , chemokine , platelet factor 4 , cytotoxic t cell , microbiology and biotechnology , immune system , immunology , pi3k/akt/mtor pathway , signal transduction , platelet , biochemistry , in vitro
We provide evidence that platelet factor 4 (PF4), but not the related chemokine neutrophil‐activating polypeptide‐2, induced highly purified human natural killer (NK) cells to produce interleukin (IL)‐8 in a time‐ and dosage‐dependent manner. This ability was retained even while PF4 was bound to heparin. PF4 increased the steady state level of IL‐8 mRNA, likely implying a transcriptional effect of PF4. Stimulation of NK cells through the Fc receptor for immunoglobulin G‐IIIA was foud to synergistically increase the effect of PF4 on IL‐8 production but did not affect IL‐2‐related activities such as cytotoxic activity and proliferation. Pertussis toxin did not block the PF4‐derived IL‐8 production in NK cells, but this response was sensitive to wortmannin, implicating a role of phosphatidylinositol 3‐kinase in the intracellular signaling pathway triggered by PF4. Our results characterize a new capacity for PF4 and provide further evidence for the pivotal role of NK cells in the environment of inflammation.