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CpG‐DNA stimulates cellular and humoral immunity and promotes Th1 differentiation in aged BALB/c mice
Author(s) -
Maletto Belkys,
Rópolo Andrea,
Morón Victor,
PistoresiPalencia María Cristina
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.72.3.447
Subject(s) - biology , immunology , immune system , cpg site , antigen , ovalbumin , antibody , dna vaccination , microbiology and biotechnology , dna methylation , immunization , gene expression , gene , biochemistry
We examined whether CpG‐DNA could be used as adjuvant to induce a T helper cell type‐1 (Th1) immunity in aged BALB/c mice that showed a Th2 polarization. Bordetella pertussis and complete Freund's adjuvant (CFA) were used as well. Immunization with ovalbumin (OVA)/CpG‐DNA showed that the immunoglobulin G (IgG)2a/IgG1 ratio and OVA‐specific T cell response were similar in young and aged mice. OVA/CpG‐DNA induced the secretion of interferon‐γ (IFN‐γ) and absence of interleukin (IL)‐5. Similar results were found in mice immunized with OVA/CFA. When mice were immunized with OVA/ B. pertussis , we found that the IgG2a/IgG1 ratio and OVA‐specific T cell response were lower in aged mice and elicited IFN‐γ and IL‐5. In vitro CpG‐DNA stimulated antigen‐presenting cells to display IL‐12 and up‐regulate the expression of major histocompatibility complex class II and B7‐2 on B cells as efficiently in aged as in young mice, but the up‐regulation of B7‐1 was stronger i aged mice. The findings demonstrate that CpG‐DNA is able to induce a young‐like Th1 specific immune response in aged mice.