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Population analysis of CD4+ T cell chemokine receptor transcript expression during in vivo type‐1 (mycobacterial) and type‐2 (schistosomal) immune responses
Author(s) -
Chiu BoChin,
Shang XiaoZhou,
Stolberg Valerie R.,
Komuniecki Eric,
Chensue Stephen W.
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.72.2.363
Subject(s) - c c chemokine receptor type 6 , biology , c c chemokine receptor type 7 , cxcr5 , xcl2 , chemokine receptor , ccr4 , cc chemokine receptors , cxcr3 , microbiology and biotechnology , ccl21 , chemokine , immunology , immune system
Chemokine receptor transcripts were defined among CD4+ T cells in lymph nodes of mice with type‐1 and type‐2 inflammation, respectively, elicited by mycobacterial and schistosomal Ag. CXCR3 and CCR6 transcripts were biased to type‐1, and CCR4 transcripts increased in type‐1 and type‐2 populations. CCR3 and CCR5 signals were too weak to establish differences. CCR8 transcripts were not increased among unstimulated populations. Compared to naïve, type‐1 and type‐2 populations had reduced CCR7 and enhanced CXCR5 transcripts, consistent with a shift to memory cells. Subset depletion revealed that transcript expression was induced among CD44+ memory T cells. Surprisingly, CCR3 transcripts were enriched among CD44lo fractions. Ag stimulation augmented CXCR3, CCR4, and CCR8 but down‐regulated CCR6 and CXCR5. CCR4 showed association with IFN‐γ‐ and IL‐4‐producing cells, but other receptor transcripts were expressed among IFN‐γ/IL‐4 negative memory T cells. These studies provide several novel findings regarding Th cell chemokine receptor expression in vivo.