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Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM‐CSF have the capacity to activate IgD− B cells through direct cellular interaction
Author(s) -
Hirohata Shunsei,
Yanagida Tamiko,
Tomita Tetsuya,
Yoshikawa Hideki,
Ochi Takahiro
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.6.987
Subject(s) - bone marrow , progenitor cell , immunology , biology , immunoglobulin d , cd19 , cd34 , b cell , stem cell , microbiology and biotechnology , stem cell factor , antibody
Recent studies have suggested the involvement of bone marrow in the pathogenesis of rheumatoid arthritis (RA), in which proliferation of monocyte‐lineage cells (MLC) as well as local B cell activation in the synovium play an important role. Here, we show that bone marrow‐derived MLC have the capacity to activate human peripheral blood IgD− B cells. Bone marrow CD34+ cells from RA patients that had been stimulated with stem cell factor and GM‐CSF for 3–4 weeks (>90% CD14+ HLA‐DR+ cells, <0.5% CD19+ B cells, and <0.5% CD3+ T cells; MLC) induced the production of IgG much more effectively than that of IgM by highly purified B cells from healthy donors in the presence of IL‐2 and IL‐10. CD34+ cells from cord blood or from bone marrow of osteoarthritis patients also displayed the capacity to induce IgG production. The induction of IgG production by the bone marrow‐derived MLC was markedly decreased when they were separated from B cells by a membrane filter. The bone marrow‐derived MLC interacted preferentially with IgD− B cells to induce IgG production. These results indicate that upon stimulation with stem cell factor and GM‐CSF, CD34+ progenitor cells differentiate into MLC that activate preferentially IgD− B cells through direct cellular interactions to produce IgG. Therefore, the data suggest that the accelerated recruitment of MLC from the bone marrow to the synovium might play a role in the local B cell activation in RA.