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Potentiation by human serum of anti‐inflammatory cytokine production by human macrophages in response to apoptotic cells
Author(s) -
Kurosaka Kahori,
Watanabe Naoko,
Kobayashi Yoshiro
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.6.950
Subject(s) - proinflammatory cytokine , cytokine , phagocytosis , apoptosis , biology , macrophage , immune system , inflammation , immunology , monocyte , microbiology and biotechnology , in vitro , biochemistry
Phagocytosis of apoptotic cells by macrophages leads to the production of anti‐inflammatory cytokines, thereby preventing inflammation. In this study, we demonstrate that human serum potentiates the production of anti‐inflammatory cytokines, IL‐10 and TGF‐β, by PMA‐treated THP‐1 cells and human monocyte‐derived macrophages in response to apoptotic cells, which results in great suppression of the production of proinflammatory cytokine IL‐8. Human IgG but not its F(ab)′ 2 suppressed the IL‐8 production. Pretreatment of macrophages but not apoptotic cells with human serum or human IgG caused the suppression, suggesting that immune complex may not be formed with apoptotic cells. When FcγRI was specifically down‐modulated by a monoclonal antibody, M22, the potentiating effects of human serum and human IgG on the anti‐inflammatory cytokine production and the suppressive effects on IL‐8 production were completely abolished. Thus, human IgG and FcγRI appear to be critical in leading to the production of anti‐inflammatory cytokines by macrophage in response to apoptotic cells.