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Flt‐3 ligand (FL) drives differentiation of rat bone marrow‐derived dendritic cells expressing OX62 and/or CD161 (NKR‐P1)
Author(s) -
BrissetteStorkus Cynthia S.,
Kettel J. C.,
Whitham T. F.,
GiezemanSmits K. M.,
Villa L. A.,
Potter D. M.,
Chambers William H.
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.6.941
Subject(s) - biology , bone marrow , cd86 , mhc class ii , microbiology and biotechnology , dendritic cell , cd80 , cytokine , antigen , immunology , in vitro , cd40 , major histocompatibility complex , immune system , t cell , cytotoxic t cell , biochemistry
Bone marrow‐derived dendritic cells (DC) of the rat have not been as well characterized as those from the mouse. Here, large quantities of bone marrow‐derived rat DC were generated when Flt‐3 ligand (FL) was used as an adjunct to granulocyte macrophage‐colony stimulating factor (GM‐CSF) and interleukin‐4 (IL‐4). These cells displayed a typical DC phenotype, expressing MHC class II, CD54, CD80, CD86, and CD11b/c. These DC also uniformly expressed low levels of CD161 and expressed OX62 in a bimodal distribution. Few cells were recovered from cultures grown without FL, and they failed to express OX62 or CD161. The DC generated with FL were more potent antigen‐presenting cells in mixed lymphocyte cultures than cells grown without FL, and among FL‐derived cells, the OX62 + cells were slightly more stimulatory than OX62 − cells. Thus, FL is a useful cytokine for obtaining large quantities of functional rat DC subsets in vitro.