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Signaling to localized degranulation in neutrophils adherent to immune complexes
Author(s) -
Nauclér Claes,
Grinstein Sergio,
Sundler Roger,
Tapper Hans
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.4.701
Subject(s) - azurophilic granule , degranulation , biology , microbiology and biotechnology , syk , protein kinase c , secretion , cytochalasin b , tyrosine kinase , kinase , signal transduction , biochemistry , receptor , immunology , myeloperoxidase , inflammation , cell
The present study demonstrates that the secretion of azurophilic granules occurring during Fc receptor‐mediated attachment and spreading of neutrophils is highly localized to the adhering region of the cell. In contrast, the secretion of specific granules occurs in a nonpolarized way. This implies that unique signals are involved in the regulation of azurophilic degranulation. Assembly of actin filaments, as visualized by staining with rhodamine phalloidin, neither hindered nor facilitated degranulation. Further, the azurophilic secretory response remained localized in the presence of cytochalasin B. Release of azurophilic‐granule content was inhibited by genistein and erbstatin, inhibitors of tyrosine kinases, and by GF109203X, a protein kinase C (PKC) inhibitor. We could also demonstrate a relative enrichment of syk tyrosine kinase and the PKC isoforms α and β1 in adherent plasma membranes.