Premium
IFN‐α2a induces IP‐10/CXCL10 and MIG/CXCL9 production in monocyte‐derived dendritic cells and enhances their capacity to attract and stimulate CD8 + effector T cells
Author(s) -
Padovan Elisabetta,
Spagnoli Giulio C.,
Ferrantini Maria,
Heberer Michael
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.4.669
Subject(s) - biology , microbiology and biotechnology , cytotoxic t cell , chemokine , cxcl10 , cd8 , c c chemokine receptor type 7 , cxcr3 , effector , dendritic cell , cytokine , chemokine receptor , immunology , immune system , in vitro , biochemistry
Type I IFNs are immunomodulatory factors that possibly influence the properties of tissue‐resident dendritic cells. Here, we have investigated the capacity of IFN‐α2a to enhance DC chemoattractive and stimulatory capacity toward CD8 + T lymphocytes. Phenotypically, IFN‐α2a‐treated DC (IFN‐DC) showed an increased expression of costimulatory and antigen‐presenting molecules, maintained even after withdrawal of the cytokine. IFN‐α2a enhanced DC stimulatory capacity toward CD8 + T cells, as assessed by increased MLR responses and induction of MART‐1 26–35 ‐specific CTLs in vitro. No functional CCR7 chemokine receptor could be induced. Instead, high amounts of IP‐10/CXCL10 and MIG/CXCL9 chemokines were produced. Freshly isolated CD8 + RO + cells and PHA‐activated CD8 + T cells migrated efficiently in response to IFN‐DC‐conditioned medium, and the migration could be inhibited by neutralizing the CXCR3 receptor on responder cells. These results suggest that type I IFNs could enhance the elicitation of class I‐restricted effector functions in vivo in the periphery by modulating DC chemoattractive properties.