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Physiological levels of 1α, 25 dihydroxyvitamin D3 induce the monocytic commitment of CD34+ hematopoietic progenitors
Author(s) -
Grande Alexis,
Montanari Monica,
Tagliafico Enrico,
Manfredini Rossella,
Marani Tommaso Zanocco,
Siena Michela,
Tenedini Elena,
Gallinelli Andrea,
Ferrari Sergio
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.4.641
Subject(s) - haematopoiesis , biology , cd34 , cd38 , progenitor cell , microbiology and biotechnology , cord blood , stem cell , immunology
Although supraphysiological levels of 1α, 25 dihydroxyvitamin D3 (VD) have been demonstrated extensively to induce the monomacrophagic differentiation of leukemic myelo‐ and monoblasts, little is known about the role that physiological levels of this vitamin could play in the regulation of normal hematopoiesis. To clarify this issue, we adopted a liquid‐culture model in which cord blood CD34+ hematopoietic progenitors, induced to differentiate in the presence of different combinations of cytokines, were exposed to VD at various concentrations and stimulation modalities. The data obtained show that physiological levels of VD promote a differentiation of CD34+ hematopoietic progenitors characterized by the induction of all the monomacrophagic immunophenotypic and morphological markers. This effect is not only exerted at the terminal maturation but also at the commitment level, as demonstrated by the decrease of highly undifferentiated CD34+CD38− hematopoietic stem cells, the down‐regulation of CD34 antigen, and the increase of monocyte‐committed progenitors. Molecular analysis suggests that the VD genomic signaling pathway underlies the described differentiation effects.