Premium
The synthetic chemoattractant peptide, Trp‐Lys‐Tyr‐Met‐Val‐ d ‐Met, enhances monocyte survival via PKC‐dependent Akt activation
Author(s) -
Bae YoeSik,
Kim Youndong,
Park Jun Chul,
Suh PannGhill,
Ryu Sung Ho
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.2.329
Subject(s) - protein kinase b , ly294002 , mapk/erk pathway , monocyte , protein kinase c , microbiology and biotechnology , pi3k/akt/mtor pathway , biology , signal transduction , immunology
Previously, we showed that Trp‐Lys‐Tyr‐Met‐Val‐ d ‐Met(WKYMVm) stimulates superoxide generation and chemotactic migration inmonocytes and neutrophils. In this study, we examined the effect of WKYMVm on monocyte survival. Serum starvation‐induced monocyte deathwas attenuated in the presence of WKYMVm, which was abated when thecells were preincubated with LY294002, suggesting the involvement ofphosphoinositide‐3‐kinase (PI 3‐kinase) in the peptide‐induced monocytesurvival. WKYMVm stimulated ERK and Akt activity via PI 3‐kinaseactivation in monocytes. We also investigated the signaling pathway of WKYMVm‐induced ERK and Akt activation. The WKYMVm‐induced ERKactivation was PI 3‐kinase‐dependent but PKC‐independent. However, Aktactivation by WKYMVm was dependent not only on PI 3‐kinase but also onthe PKC pathway. When monocytes were incubated with WKYMVm, caspase‐3activity, which is important for cell death, was inhibited. Pretreatment of the cells with LY294002, GF109203X, and Go 6976 but notPD98059 blocked WKYMVm‐induced monocyte survival and caspase‐3inhibition. In summary, the novel chemoattractant WKYMVm enhancesmonocyte survival via Akt‐mediated pathways, and in this process, PKCand PI 3‐kinase act upstream of Akt.