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(−)Epigallocatechin‐3‐gallate inhibits leukocyte elastase: potential of the phyto‐factor in hindering inflammation, emphysema, and invasion
Author(s) -
Sartor Luigi,
Pezzato Elga,
Garbisa Spiridione
Publication year - 2002
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.71.1.73
Subject(s) - cathepsin g , elastase , pancreatic elastase , biology , thrombin , inflammation , neutrophil elastase , epigallocatechin gallate , cathepsin , pharmacology , immunology , biochemistry , enzyme , polyphenol , platelet , antioxidant
Flavanol (−)epigallocatechin‐3‐gallate is shown to be a potent naturalinhibitor of leukocyte elastase that may be used to reduceelastase‐mediated progression to emphysema and tumor invasion. Thisphyto‐factor, abundant in green tea, exerts a dose‐dependent, noncompetitive inhibition of leukocyte elastase at a noncytotoxicconcentration and is effective in neutrophil culture. This inhibitionshows an IC 50 of 0.4 μM, 30 times higher than theα1‐protease inhibitor but lower than other known natural andsynthetic elastase inhibitors. The flavanol inhibits leukocyte elastaseat concentrations of 50, 150, and 2500 times lower than that effectiveon gelatinases (MMP‐2 and MMP‐9), thrombin, and cathepsin G, respectively, and also blocks elastase‐mediated activation of MMP‐9.