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Intercellular cell adhesion molecule‐1 regulates lymphocyte movement into intestinal microlymphatics of rat Peyer’s patches
Author(s) -
Hokari Ryota,
Miura Soichiro,
Nagata Hiroshi,
Fujimori Hitoshi,
Koseki Seiichiro,
Kato Shingo,
Kurose Iwao,
Sekizuka Eiichi,
Granger D. Neil,
Ishii Hiromasa
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.6.896
Subject(s) - biology , intracellular , microbiology and biotechnology , lymphocyte , adhesion , cell adhesion molecule , intercellular adhesion molecule 1 , peyer's patch , cell adhesion , t lymphocyte , immunology , cell , lymphatic system , immune system , genetics , chemistry , organic chemistry
The objective of this study was to determine whether specific adhesion molecules modulate lymphocyte movement from Peyer’s patches into intestinal microlymphatics. The fluorochrome acridine orange was injected via a micropipette into Peyer’s patches to fill lymphatics. The flux of labeled lymphocytes into intestinal microlymphatics was monitored with intravital fluorescence microscopy. The lymphatic microvessels in the perifollicular area of Peyer’s patches were filled with lymphocytes, most of which remained within the lymphatics. Some lymphocytes became detached and were drained into intestinal lymph. Administration of antibodies directed against ICAM‐1 significantly increased lymphocyte flux into interfollicular lymphatics. The immunohistochemical study showed intense ICAM‐1 expression on the lymphocytes densely packed in the lymphatics surrounding follicles in Peyer’s patches. A large number of lymphocytes are normally sequestered in the lymphatic network of Peyer’s patches. This sequestration of lymphocytes is largely mediated by ICAM‐1‐dependent cell‐cell interactions.