Premium
C1qR p , a myeloid cell receptor in blood, is predominantly expressed on endothelial cells in human tissue
Author(s) -
Fonseca Maria I.,
Carpenter Philip M.,
Park Minha,
Palmarini Gail,
Nelson Edward L.,
Tenner Andrea J.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.5.793
Subject(s) - biology , microbiology and biotechnology , receptor , polyclonal antibodies , cell type , in vitro , opsonin , dendritic cell , endothelial stem cell , myeloid , antibody , cell , immunology , antigen , phagocytosis , biochemistry
C1qR p is a type I cell surface glycoprotein that has been shown to enhance ingestion of suboptimally opsonized targets by phagocytes in vitro. In this study, we developed and characterized polyclonal antibodies to study the tissue distribution of this receptor targeted to either the N‐ or C‐terminal portion of the molecule. C1qR p was detected in vascular endothelial cells and in a subset of pyramidal neurons in the brain, as well as neutrophils, but it was absent in most tissue macrophages. Analysis of in vitro differentiation of blood monocytes to dendritic cells demonstrated a down‐regulation of the receptor as monocytes differentiate to dendritic cells, providing a possible explanation for the lack of reactivity of these cells in tissue. The predominant presence of C1qR p in endothelial cells, while compatible with a phagocytic role in host defense and/or clearance of cellular material, suggests other possible novel roles for this receptor.