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A cytokine‐to‐chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases
Author(s) -
Albanesi Cristina,
Scarponi Claudia,
Sebastiani Silvia,
Cavani Andrea,
Federici Monica,
Sozzani Silvano,
Girolomoni Giampiero
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.4.617
Subject(s) - ccl17 , ccl5 , chemokine , cxcl10 , ccl2 , biology , chemotaxis , immunology , ccl13 , keratinocyte , ccl22 , cytokine , microbiology and biotechnology , ccl20 , lymphokine , t cell , inflammation , cell culture , immune system , il 2 receptor , chemokine receptor , biochemistry , genetics , receptor
The recruitment of T cells into the skin is regulated by chemokines released by resident cells. In this study, we found that normal human keratinocytes activated with Th1‐derived supernatant (sup) expressed early (6–12 h) IP‐10/CXCL10, MCP‐1/CCL2, IL‐8/CXCL8, and I‐309/CCL1 mRNAs and with slower kinetics (24–96 h), RANTES/CCL5 and MDC/CCL22 mRNAs. Upon stimulation with the Th1 sup, keratinocytes secreted high levels of RANTES, IP‐10, MCP‐1, and IL‐8 and moderate levels of I‐309 and MDC. Although much less efficiently, Th2 sup could also induce keratinocyte expression of IL‐8, IP‐10, RANTES, and MCP‐1 but not of I‐309 and MDC. TARC/CCL17 was not significantly induced by any stimuli. Sup from keratinocytes activated with Th1‐derived cytokines elicited a strong migratory response of Th1 cells and a limited migration of Th2 cells, whereas sup from Th2‐activated keratinocytes promoted a moderate migration of Th1 and Th2 lymphocytes. Thus, keratinocytes appear considerably more sensitive to Th1‐ than to Th2‐derived lymphokines in terms of chemokine release and can support the preferential accumulation of Th1 lymphocytes in the skin.