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CCR1 chemokine receptor expression isolates erythroid from granulocyte‐macrophage progenitors
Author(s) -
de Wynter Erika A.,
Heyworth Clare M.,
Mukaida Naofumi,
Jaworska Ewa,
WeffortSantos Almeriane,
Matushima Kouji,
Testa Nydia G.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.3.455
Subject(s) - ccr1 , progenitor cell , biology , haematopoiesis , chemokine receptor , cd34 , immunology , granulocyte , microbiology and biotechnology , chemokine receptor ccr5 , cc chemokine receptors , cord blood , progenitor , chemokine , stem cell , immune system
Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34 + cells were separated into CD34 + CCR1 + and CD34 + CCR1 − cells and plated in colony‐forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34 + CCR1 + cells. In contrast, the CD34 + CCR1 − cells contained the majority of the erythroid progenitors. There was a highly significant difference ( P <0.002) in the total percentage distribution of both granulocyte‐macrophage colony‐forming cells and erythroid burst‐forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage‐specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation.