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Effects of hypertonic saline on expression of human polymorphonuclear leukocyte adhesion molecules
Author(s) -
Thiel M.,
Buessecker F.,
Eberhardt K.,
Chouker A.,
Setzer F.,
Kreimeier U.,
Arfors K.E.,
Peter K.,
Messmer K.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.2.261
Subject(s) - hypertonic saline , tonicity , biology , protein kinase c , endocrinology , medicine , n formylmethionine leucyl phenylalanine , receptor , biochemistry , signal transduction , chemotaxis
Hypertonic saline prevents vascular adherence of neutrophils and ameliorates ischemic tissue injury. We hypothesized that hypertonic saline attenuates N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP)‐stimulated expression of adhesion molecules on human polymorphonuclear leukocytes (PMNLs). fMLP‐stimulated up‐regulation of β2‐integrins was diminished by hypertonic saline but not by hypertonic choline chloride‐, mannitol‐, or sucrose‐modified Hanks’ buffered salt solution. Shedding of l ‐selectin was decreased by hypertonic saline and choline chloride but not by hypertonic mannitol or sucrose. When the effects of hypertonic sodium chloride‐ and choline chloride‐modified media were compared, neither solution affected fMLP‐receptor binding but both equally inhibited fMLP‐stimulated increase in intracellular calcium, ionophore A23187, and phorbol myristate acetate (PMA)‐stimulated numerical up‐regulation of β2‐integrins. Analysis of mitogen‐activated protein (MAP) kinases p38 and p44/42 for phosphorylation revealed that hypertonic solutions did not differ in preventing fMLP‐stimulated increases in phospho‐p38 and phospho‐p44/42. Resting PMNLs shrunk by hypertonic saline increased their volume during incubation and further during chemotactic stimulation. Addition of amiloride further enhanced inhibition of up‐regulation of β2‐integrins. No fMLP‐stimulated volume changes occurred in PMNLs exposed to hypertonic choline chloride, resulting in significant cell shrinkage. Results suggest a sodium‐specific inhibitory effect on up‐regulation of β2‐integrins of fMLP‐stimulated PMNLs, which is unlikely to be caused by alterations of fMLP receptor binding, decrease in cytosolic calcium, attenuation of calcium or protein kinase C‐dependent pathways, suppression of p38‐ or p44/42 MAP kinase‐dependent pathways, or cellular ability to increase or decrease volumes.