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Growth factor regulation of neutrophil‐endothelial cell interactions
Author(s) -
Zhang Hong,
Issekutz Andrew C.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.2.225
Subject(s) - biology , microbiology and biotechnology , immunology , endothelial stem cell , genetics , in vitro
The effects of the angiogenic factors basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) on human polymorphonuclear leukocyte (PMNL)‐endothelial cell adhesion and transendothelial migration (TEM) were investigated. Stimulation of human umbilical vein endothelial cells by VEGF or bFGF for 18 h up‐regulated intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 expression and significantly increased PMNL adhesion and TEM in response to complement fragment 5a (C5a) or interleukin (IL)‐8. In contrast, continued exposure to bFGF (24 h–6 days) down‐regulated basal and IL‐1‐ or tumor necrosis factor (TNF)‐induced intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and E‐selectin expression as well as PMNL adhesion and TEM. These effects could be reversed by introduction of high concentrations of TNF‐α, C5a, or IL‐8. None of these inhibitory effects was observed with VEGF. The acute effects of bFGF and VEGF may facilitate PMNL emigration during acute inflammation, but continued bFGF production may have anti‐inflammatory actions during chronic inflammation, angiogenesis, and tumor defense by inhibition of endothelial activation for leukocyte recruitment.