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Chemokine receptors in human basophils: inducible expression of functional CXCR4
Author(s) -
Iikura Motoyasu,
Miyamasu Misato,
Yamaguchi Masao,
Kawasaki Hiroshi,
Matsushima Kouji,
Kitaura Motoji,
Morita Yutaka,
Yoshie Osamu,
Yamamoto Kazuhiko,
Hirai Koichi
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.70.1.113
Subject(s) - chemokine receptor , ccr3 , cxc chemokine receptors , ccl13 , biology , chemokine , receptor , cxcr4 , cc chemokine receptors , ccr2 , ccr1 , eotaxin , ccl21 , immunology , microbiology and biotechnology , biochemistry
We examined the expression profile of chemokine receptors in humanbasophils and their regulation by cytokines. Basophils expressedtranscripts of CC chemokine receptors (CCR)1, CCR2, CCR3, and CCR5 andCXC chemokine receptors (CXCR)1, CXCR2, and CXCR4. In contrast to theother receptors, surface‐CXCR4 expression was not detected in fresh‐and whole‐blood basophils, but it became apparent gradually duringincubation. Among 16 chemokines tested, eotaxin induced the most potentbasophil migration. SDF‐1 also induced a strong, migratory responsecomparable with that induced by eotaxin in 24‐h, cultured basophils,but it failed to induce degranulation. IL‐3 abrogated CXCR4 expressioncompletely, and it only down‐regulated CCR2 and CCR3 expressionslightly. IL‐5, GM‐CSF, and IL‐4 also down‐regulated CXCR4 expression.Thus, expression of CXCR4 was the most strongly affected by cytokines,and this may represent an alternative mechanism for control ofcell‐specific, biological responses to SDF‐1.