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Expression, tissue distribution, and cellular localization of the antiapoptotic TIP‐B1 protein
Author(s) -
Berleth Erica S.,
MassoWelch Patricia A.,
Kazim Latif A.,
Ip Margot M.,
Mihich Enrico,
Ehrke M. Jane
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.6.995
Subject(s) - biology , tumor necrosis factor alpha , spleen , lymph node , immunohistochemistry , apoptosis , microbiology and biotechnology , pathology , endocrinology , immunology , medicine , biochemistry
TIP‐B1 is a novel 27‐kDa protein isolated from the cytosol of tumornecrosis factor (TNF)‐stimulated cells. Cells preincubated with TIP‐B1are protected from TNF‐induced apoptosis. This study showed that, aswith normal fibroblasts and U937 histiocytic lymphoma, human MCF7mammary adenocarcinoma cells were protected from TNF in aconcentration‐dependent manner by pretreatment with either TNF orpurified TIP‐B1. Immunoblot and immunohistochemical analyses indicatedexpression of both TIP‐B1 mRNA and protein in MCF7 cells and heart, kidney, brain, liver, ovary, uterus, thymus, spleen, lymph node, andmammary gland cells throughout their development. Expression of TIP‐B1was heterogeneous, with staining of specific cell types within tissues. Based on the ability of TIP‐B1 to protect both normal and tumor cellsfrom TNF‐induced apoptosis and its broad tissue distribution, withexpression only in select cells within those tissues, a role for TIP‐B1in the regulation of TNF‐induced effects is strongly indicated.