Premium
Differences in splenic B‐lymphocyte ganglioside expression and accessibility in normal and endotoxin‐hyporesponsive mice
Author(s) -
Berenson Charles S.,
Rasp Robin H.,
Gau JenTzer,
Ryan John L.,
Yohe Herbert C.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.6.969
Subject(s) - panning (audio) , ganglioside , biology , antibody , microbiology and biotechnology , lymphocyte , immunology , biochemistry , zoom , lens (geology) , paleontology
Endotoxin‐responsive (C3H/HeN) and ‐hyporesponsive (C3H/HeJ) murine Blymphocytes purified by adherence to anti‐immunoglobulin (“antibodypanning”) possess identical gangliosides but different gangliosidesurface accessibilities. We investigated the distribution and surfaceaccessibility of gangliosides of B lymphocytes purified by adherence toplastic (“plastic panning”) or by subtraction of non‐B‐lymphocytecomponents. As with antibody panning, there were no entirely new orabsent gangliosides in plastic‐panned or subtraction‐purified Blymphocytes of each strain. However, striking changes in relativeexpression of five gangliosides were detected with each purificationprotocol. Moreover, five gangliosides of antibody‐panned andplastic‐panned B lymphocytes but only two gangliosides ofsubtraction‐purified B lymphocytes were inaccessible to surfacelabeling. Unlike the situation for antibody‐panned B lymphocytes, nointerstrain (HeN vs. HeJ) surface accessibility differences existed ingangliosides of plastic‐panned or subtraction‐purified cells. Exposureof subtraction‐purified B lymphocytes to anti‐immunoglobulin failed toelicit changes in ganglioside expression. Murine B lymphocytes havedistinct protocol‐dependent differences in glycolipid phenotypewhich likely denote individual subpopulations.