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Tissue distribution of CD6 and CD6 ligand in cattle: expression of the CD6 ligand (CD166) in the autonomic nervous system of cattle and the human
Author(s) -
Konno A.,
Ahn J.S.,
Kitamura H.,
Hamilton M. J.,
Gebe J. A.,
Aruffo A.,
Davis W. C.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.6.944
Subject(s) - biology , immunohistochemistry , monoclonal antibody , ligand (biochemistry) , microbiology and biotechnology , spleen , antibody , receptor , immunology , biochemistry
We studied the tissue distribution of CD6 + lymphocytes and cells expressing the CD6 ligand (also known as activated leukocyte cell adhesion molecule CD166) in calves by immunohistochemistry using an anti‐bovine CD6 monoclonal antibody (mAb), a human CD6 (huCD6)‐immunoglobulin G1 fusion protein (huCD6‐Ig), and an anti‐human CD166 (anti‐huCD166) mAb. The huCD6‐Ig and anti‐huCD166 mAb bound to the sympathetic and parasympathetic nerve fibers but not to myelinated nerve fibers in the spinal nerve. Studies with human tissue using the anti‐huCD166 mAb yielded identical patterns of labeling. Dense accumulations of CD6 + lymphocytes were present in areas of the thymuses and spleens of calves, in areas innervated by huCD6‐Ig + nerves. The cDNAs encoding the bovine CD166 and CD6 were isolated from the sympathetic ganglion and spleen, respectively. Predicted amino acid residues that are important for human and mouse CD6‐CD166 binding were also conserved in bovine CD6 and CD166. Bovine CD166 transcripts were detected by reverse transcriptase‐PCR in all the tissues that bound huCD6‐Ig. These results show that the bovine orthologue of CD166 was constitutively expressed in the autonomic nervous systems of cattle and suggest that CD6 + lymphocytes adhere to CD166 + autonomic nerve terminals via CD6.