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Protein kinase C θ is expressed in mast cells and is functionally involved in Fcɛ receptor I signaling
Author(s) -
Liu Yin,
Graham Caroline,
Parravicini Valentino,
Brown Martin J.,
Rivera Juan,
Shaw Stephen
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.5.831
Subject(s) - lyn , protein kinase c , biology , microbiology and biotechnology , mast cell , interleukin 33 , signal transduction , kinase , receptor , immune system , immunology , proto oncogene tyrosine protein kinase src , cytokine , interleukin , biochemistry
We investigated possible expression and function in mast cells ofprotein kinase C (PKC) θ, a member of the PKC family withdemonstrated function in a limited range of cell types. We found thatPKC θ is expressed in bone marrow‐derived mast cells and in the RBL‐2H3 mast cell line. PKC θ underwent translocation to the membranein response to Fcɛ receptor I (FcɛR I) activation. Receptoractivation induced phosphorylation of PKC θ. The tyrosinephosphorylation of PKC θ is delayed relative to PKC δ and coincidestemporally with PKC θ association with c‐src family members Lyn andSrc. Studies of RBL‐2H3 cells transduced with PKC θ constructsindicated a role for PKC θ in receptor‐induced activation ofextracellular regulated kinases, interleukin‐3 gene transcription, anddegranulation in response to antigen stimulation. These studies extendthe known functions of PKCθ to another important immune cell type andindicate the concurrent participation of multiple PKCs in the FcɛRI‐mediated response of mast cells.