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Changes of HSP72‐expression in leukocytes are associated with adaptation to exercise under conditions of high environmental temperature
Author(s) -
Fehrenbach Elvira,
Niess Andreas Michael,
Veith Roman,
Dickhuth HansHerrmann,
Northoff Hinnak
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.5.747
Subject(s) - biology , adaptation (eye) , expression (computer science) , microbiology and biotechnology , immunology , ecology , neuroscience , programming language , computer science
Overexpression of the heat shock protein HSP72 providesthermotolerance. We asked if two consecutive endurance runs 1 weekapart (CR1, CR2) and additional environmental heat stress affectHSP72‐expression in leukocytes of nonheat‐acclimated enduranceathletes. Twelve subjects were allocated randomly into two groups. Group HH completed both runs at 28°C ambient temperature, and groupNH performed CR1 at 18°C and CR2 at 28°C. HSP72‐expression wasdetermined by flow cytometry and RT‐PCR before and 0, 24, and 48 hafter exercise. Additionally, post‐exercise cells were exposed to in vitro heat shock (HS; 2 h, 42°C). The prolonged, high HSP72 protein level after CR1 in HH compared with NH may reflectthermotolerance induced by endurance exercise at high ambienttemperature. Adaptation of cardiocirculatory/thermoregulatory capacityafter CR2 in HH went along with a more rapid down‐regulation of HSP72compared with CR1. HSP72 mRNA demonstrated temperature‐related changesafter exercise. The reduced HS response in vitro after CR2may represent exercise‐related adaptation mechanisms. HSP72concentrations in leukocytes may indicate previous exercise‐ andtemperature‐related stress conditions and adaptation in immunocompetentcells.