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CD86 expression correlates with amounts of HIV produced by macrophages in vitro
Author(s) -
Wang Xiaoping,
Lewis Dorothy E.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.3.405
Subject(s) - biology , cd86 , in vivo , in vitro , basal (medicine) , immunology , human immunodeficiency virus (hiv) , macrophage , cd40 , immune system , microbiology and biotechnology , t cell , endocrinology , biochemistry , genetics , insulin , cytotoxic t cell
Primary macrophages from different donors produce variable levels of HIV; however, the mechanisms are unclear. We tested whether variations in cell‐surface or cell‐cycle characteristics influenced HIV production. We found that greater basal proliferation of the macrophages prior to infection resulted in more arrested in G 2 M 3 days post‐infection (r 2 =0.7, P <0.04). Likewise, the number of G 2 M‐arrested macrophages correlated with p24 production (r 2 =0.78, P <0.02) and apoptosis (r 2 =0.67, P <0.05) later in the infection. Serum‐starvation or reduction, which limit HIV spread, reduced G 2 M arrest and HIV amounts. Surprisingly, the amount of HIV produced correlated with expression levels of the costimulating ligand, CD86, but not with other important molecules, including class II, CD40, or CD54 (r 2 =0.96, P <0.0005). These data establish donor characteristics related to variable HIV production in vitro and suggest that altered expression of costimulatory ligands may influence HIV production in vivo.