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Differences in the induction of CD8 + T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL‐1α secretion
Author(s) -
Hope Jayne C.,
Sopp Paul,
Collins Robert A,
Howard Chris J.
Publication year - 2001
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.69.2.271
Subject(s) - biology , cd8 , microbiology and biotechnology , cytotoxic t cell , t cell , antigen presenting cell , dendritic cell , antigen , immunology , immune system , in vitro , biochemistry
The major subset of dendritic cells (DC) from bovine afferent lymph expresses the SIRPα MyD‐1 antigen, but not CD11a or the antigen recognized by mAb CC81, and potently stimulates CD4 + and CD8 + T lymphocyte proliferation. The minor subpopulation, that is CD11a + CC81 + MyD‐1 − , effectively stimulates CD4 + but not CD8 + T lymphocyte proliferation. CD11a + CC81 + MyD‐1 − DC did not induce anergy or death or secrete an inhibitory factor. However, supernatant from cultures of CD8 + T cells with CD11a − CC81 − MyD‐1 + DC significantly enhanced proliferation of CD8 + T cells in response to CD11a + CC81 + MyD‐1 − DC, an effect that was blocked by interleukin (IL)‐1α, but not IL‐1β, specific mAb. The proliferation of CD8 + T cells with CD11a + CC81 + MyD‐1 − DC was also enhanced by adding IL‐1α. IL‐1β slightly enhanced proliferation, whereas IL‐2, IL‐6, IL‐12, and IL‐15 had no effect. We conclude that the failure to stimulate CD8 + T cell proliferation results from the lack of IL‐1α synthesis by this population, which may have important consequences in vivo .