Premium
Activating Ly‐49D NK receptors: expression and function in relation to ontogeny and Ly‐49 inhibitor receptors
Author(s) -
Ortaldo John R.,
WinklerPickett Robin,
Wiegand Gordon
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.68.5.748
Subject(s) - biology , receptor , inhibitory postsynaptic potential , repertoire , ontogeny , function (biology) , flow cytometry , microbiology and biotechnology , immunology , genetics , endocrinology , physics , acoustics
Developmental changes in the repertoire of activating Ly‐49 family members have not been examined previously. In the present study, we have examined the expression and function of the activating Ly‐49s (D and H) from birth through 8 weeks of age. We demonstrate that 1) activating Ly‐49s are expressed early, 2) their expression intensity is not different from adult NK cells, and 3) activating receptors are functional. Examination of the inhibitory Ly‐49s also demonstrated functional capacity immediately upon expression. To examine the kinetics of expression of the repertoire of activating Ly‐49 members, we utilized five‐ and six‐color flow cytometric analyses of NK cells from birth through adulthood. Previous studies examining the inhibitory Ly‐49 repertoire have proposed that expression is regulated by the product rule. Our results indicated that Ly‐49D, which recognizes H‐2D d , had a discordantly high coexpression of the inhibitory Ly‐49s that recognized H‐2D d (Ly‐49A and Ly‐49G2). The product rule of Ly‐49 expression does not explain the coexpression of selected activating and inhibitory receptors. This high level of coexpression of H‐2D d recognizing activating and inhibitory Ly‐ 49s suggests an in vivo selection or regulated coexpression.