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Clustering on the forward surfaces of migrating neutrophils of a novel GPI‐anchored protein that may regulate neutrophil adherence and migration
Author(s) -
NakamuraSato Yukiko,
Sasaki Katsunori,
Watanabe Hiroshi,
Araki Yoshihiko,
Sendo Fujiro
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.68.5.650
Subject(s) - biology , neutrophil extracellular traps , microbiology and biotechnology , glycoprotein , stimulation , immunology , extravasation , inflammation , neuroscience
We previously reported a novel glycosylphosphatidylinositol (GPI)‐anchored glycoprotein (tentatively designated GPI‐80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno‐ and scanning electron microscopy, the distribution of GPI‐80 on neutrophil surfaces. GPI‐80 was diffusely distributed on the surface of resting neutrophils and on the peripheral areas of adherent cells after stimulation with N ‐formyl‐methionyl‐leucyl‐phenylalanine. After longer stimulation (60 min), GPI‐80 decreased in number and was again diffusely distributed on the surfaces of round neutrophils. Few GPI‐80 were detected on the ventral surfaces of adherent neutrophils. Clusters of GPI‐80 were detected on the forward surfaces of neutrophils transmigrating through pores of nitrocellulose membranes. These results may give a morphological background of possible role of GPI‐80 for neutrophil extravasation.