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Recognition of CHO cells by inhibitory and activating Ly‐49 receptors
Author(s) -
Mason Llewellyn H.
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.68.4.583
Subject(s) - receptor , biology , chinese hamster ovary cell , microbiology and biotechnology , inhibitory postsynaptic potential , ligand (biochemistry) , immunology , endocrinology , biochemistry
Upon ligand recognition, members of the murine Ly‐49 receptor family can transmit inhibitory or activating signals that regulate NK cell function. Ly‐49A, G, and D have been shown to recognize the murine class I molecule H‐2D d as a potential ligand. Recent studies also have demonstrated also that Ly‐49D + NK cells can lyse CHO cells, although the ligand responsible for this recognition was not identified. Because allorecognition by NK cells may be important in bone‐marrow transplantation and because of the overlapping class I recognition by these receptors, recognition of CHO cells by Ly‐49G and A was investigated. Our data suggest that Ly‐49G and probably A transmit inhibitory signals in response to CHO cells. Receptor inhibition was assessed by examining NK lytic function, IFN‐γ secretion, and DAP12 phosphorylation in response to CHO cells by sorted subsets of Ly‐49D vs. G B6 NK cells. Our results suggest that CHO cells may express a common ligand(s) that is capable of engaging Ly‐49D, G, and possibly A in C576BL/6 NK cells. In addition to our findings that Ly‐49 inhibitory receptors also recognize CHO cells, activating receptors other than Ly‐49D are present in B6 mice that can lyse CHO cells.