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IL‐4 production by human polymorphonuclear neutrophils
Author(s) -
Brandt Eric,
Woerly Gaëtane,
Younes Amena Ben,
Loiseau Sylvie,
Capron Monique
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.68.1.125
Subject(s) - secretion , biology , granulocyte , ionomycin , intracellular , peripheral blood mononuclear cell , interleukin 8 , cytokine , chemokine , calcium in biology , phagocytosis , ionophore , interleukin , immunology , inflammation , in vitro , endocrinology , microbiology and biotechnology , biochemistry , membrane
Polymorphonuclear neutrophils (PMN) are phagocytic cells, able to secrete a large range of cytokines, including inflammatory cytokines, chemokines, as well as the Th1 cytokines interferon‐γ (IFN‐γ) and interleukin (IL)‐12. Although PMN do not seem to express IL‐10 and IL‐13, no information exists on the ability of PMN to produce IL‐4. Therefore intracellular flow cytometry was performed in the presence or absence of Brefeldin A. Similarly to eosinophils, freshly isolated neutrophils from normal donors contained low amounts of IL‐4, which significantly increased upon culture with Brefeldin A ( P < 0001). Immunostaining performed on cytospin preparations of normal granulocytes confirmed the presence of intracellular IL‐4. Using a highly sensitive ELISA, the levels of IL‐4 secreted by cultured PMN and peripheral blood mononuclear cells (PBMC) were compared. PBMC secrete up to 60 times more IL‐4 as PMN but, in the presence of calcium ionophore, only PMN showed a slight but significant increase in IL‐4 secretion ( P < 0.05). In conclusion, we report here the presence within human PMN of intracellular IL‐4, which can at least partly be released under calcium ionophore stimulation. The relevance of this production of IL‐4 by human PMN is discussed.