Premium
Vascular endothelial cells provide T cells with costimulatory signals via the OX40/gp34 system
Author(s) -
Kunitomi Akane,
Hori Toshiyuki,
Imura Akihiro,
Uchiyama Takashi
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.68.1.111
Subject(s) - biology , umbilical vein , t cell , microbiology and biotechnology , monoclonal antibody , concanavalin a , interleukin 2 , immune system , immunology , antibody , in vitro , biochemistry
We investigated whether gp34, the ligand of OX40, expressed on EC is involved in costimulation of T cells. Normal CD4 + T cells were stimulated with anti‐CD3‐coated beads, phytohemagglutinin (PHA), or concanavalin A (Con A) in the presence or absence of irradiated human umbilical vein endothelial cells (HUVEC). Stimulation of T cells with each of these mitogens results in significant T‐cell proliferation only when HUVEC were present, and this proliferation was inhibited markedly by anti‐OX40 or anti‐gp34 monoclonal antibody (mAb). T cells cultured with HUVEC produced more interleukin (IL)‐2 than those cultured without HUVEC. The addition of anti‐IL‐2R α chain and anti‐IL‐2R β chain mAbs abolished the costimulatory effects of HUVEC. Thus, the augmentation of T‐cell proliferation appears to be attributable to increased IL‐2 production. These results suggest that gp34 expressed on HUVEC plays a role in potentiation of T‐cell immune response by providing OX40 + T cells with costimulatory signals.