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An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells
Author(s) -
Quatromoni Jon G.,
Singhal Sunil,
Bhojnagarwala Pratik,
Hancock Wayne W.,
Albelda Steven M.,
Eruslanov Evgeniy
Publication year - 2015
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.5ta0814-373
Subject(s) - biology , immune system , cell , phenotype , effector , in vivo , lung , function (biology) , cancer research , immunology , computational biology , microbiology and biotechnology , medicine , gene , biochemistry
A method to retrieve all major tumor‐associated cell populations with high cell yield, while maintaining the expression of cell surface markers and functions of isolated cells.