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Identification of CD4 int progenitors in mouse fetal spleen, a source of resident lymphoid cells
Author(s) -
Desanti Guillaume E.,
Cumano Ana,
Golub Rachel
Publication year - 2008
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1107755
Subject(s) - biology , progenitor cell , haematopoiesis , spleen , fetus , population , immunology , progenitor , microbiology and biotechnology , stem cell , genetics , pregnancy , medicine , environmental health
Hematopoiesis occurs in different tissues during adult and fetal life. Splenic hematopoiesis arises in the fetal period until the first weeks of life. We have analyzed the hematopoietic progenitor content of the fetal spleen (FS) at the embryonic days 14.5–15.5. We first demonstrate that the hematopoietic content of the FS differs largely from its fetal liver (FL) counterpart. The difference mainly concerns the distribution of the different pool of progenitors, as most of the splenic progenitors are comprised in the lineage − Sca1 − cKit lo contrary to the FL. We have divided the fetal hematopoietic pool into smaller fractions to enable characterization of the earliest lymphoid progenitors. Among the lymphoid progenitors that already represent a rare population, we were able to separate a population, respectively, enriched in B or T/NK progenitors. Lineage restriction of the different developmental intermediates was tested by clonal assays. We propose a model for fetal splenic hematopoietic progenitors and their distribution.