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CD11c/CD18: novel ligands and a role in delayed‐type hypersensitivity
Author(s) -
Sadhu Chanchal,
Ting Harold J.,
Lipsky Brian,
Hensley Kelly,
GarciaMartinez Leon F.,
Simon Scott I.,
Staunton Donald E.
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1106680
Subject(s) - cd11c , biology , ic3b , cd18 , cell adhesion molecule , integrin , cell adhesion , microbiology and biotechnology , immunology , integrin alpha m , monocyte , t cell , complement system , receptor , cell , flow cytometry , immune system , phenotype , biochemistry , gene
CD11c, a member of the leukointegrin family, is expressed prominently on tissue macrophages and dendritic cells and binds to complement fragment (iC3b), provisional matrix molecules (fibrinogen), and the Ig superfamily cell adhesion molecule, ICAM‐1. CD11c has been proposed to function in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T cell proliferation by Langerhans cells. Using assays to quantify CD11c‐mediated cell adhesion, we demonstrate that CD11c recognizes ICAM‐2 and VCAM‐1. The CD11c‐binding site on VCAM‐1 appears to be different from that used by the integrin α4. CD11c and α4β1 contributed to monocyte capture and transmigration on inflamed human aortic endothelial cells. We discovered that the anti‐mouse CD11c mAb N418 blocks CD11c binding to iC3b, ICAM‐1, and VCAM‐1. Treatment of mice with N418 reduced SRBC‐induced delayed‐type hypersensitivity significantly. CD11c appeared to contribute predominantly to the sensitization phase and somewhat less to the response to SRBC challenge. This suggests a novel role for CD11c during leukocyte recruitment, antigen uptake, and the survival of APC.