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Human monocyte‐derived dendritic cells differentiated in the presence of IL‐2 produce proinflammatory cytokines and prime Th1 immune response
Author(s) -
Sanarico Nunzia,
Ciaramella Antonio,
Sacchi Alessandra,
Bernasconi Daniela,
Bossù Paola,
Mariani Francesca,
Colizzi Vittorio,
Vendetti Silvia
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1105690
Subject(s) - biology , proinflammatory cytokine , immune system , monocyte , immunology , dendritic cell , cytokine , microbiology and biotechnology , il 2 receptor , lipopolysaccharide , interleukin 4 , interleukin 12 , t cell , in vitro , inflammation , cytotoxic t cell , biochemistry
Interleukin (IL)‐2 plays an important role in the control of the immune responses, and it is released in a variety of tissues in response to inflammatory stimuli. As monocytes and mature dendritic cells (DCs) express CD25, the high‐affinity subunit of IL‐2 receptor, we examined the effect of exogenous IL‐2 on the in vitro generation and maturation of DCs from monocytes. Human monocyte‐derived DCs (MDDCs) were generated by culturing monocytes with granulocyte macrophage‐colony stimulating factor (GM‐CSF) and IL‐4 in the presence or absence of IL‐2. The cytokine was added at the beginning and after 5 days of culture. Our findings indicate that IL‐2 does induce monocytes to differentiate into DCs with the same morphology and phenotype of that obtained in the presence of GM‐CSF and IL‐4 alone, but with some distinctive functional properties. DCs differentiated in the presence of IL‐2 secreted significantly more IL‐1β, TNF‐α, and IL‐12 p70 in response to lipopolysaccharide stimulation and induced allogeneic, naïve T cells to release a significantly higher amount of interferon‐γ if compared with DCs obtained by culturing monocytes with GM‐CSF and IL‐4. These results indicate unrecognized effects of IL‐2 on human MDDCs and suggest that an IL‐2‐rich environment during differentiation and maturation of DCs can modify their T helper cell‐inducing properties.