CTLA4‐CD80/CD86 interactions on primary mouse CD4 + T cells integrate signal‐strength information to modulate activation with Concanavalin A

The mechanisms by which concanavalin A (Con A), a lectin, activates T cells are poorly studied. A low dose of Con A is stimulatory for T cells, whereas a high dose of Con A results in suppression of proliferation and enhanced T cell death. The expression and functional roles of costimulatory receptors, CD28 and cytotoxic T‐lymphocyte antigen 4 (CTLA4), and their ligands, CD80 and CD86, on primary mouse CD4 + T cells after activation with different doses of Con A were studied. CTLA4‐CD80/CD86 interactions in this T:T cell activation model demonstrate distinct outcomes depending on the dose of Con A. CTLA4‐CD80/CD86 interactions inhibit CD4 + T cell cycling and survival after activation with a suppressive dose of Con A by increasing oxidative stress and decreasing levels of BclX L . The enhanced CD4 + T cell death with a suppressive dose of Con A is dependent on excess H 2 O 2 and nitric oxide but is independent of Fas and caspase activity. It is surprising that the increased proliferation of CD4 + T cells with a suppressive dose of Con A on blocking CTLA4‐CD80/CD86 interactions is largely interleukin (IL)‐2‐independent but is cyclosporine A‐sensitive. On activation with a stimulatory dose of Con A, CTLA4‐CD80/CD86 interactions enhance T cell activation and survival by reducing the production of reactive oxygen species, increasing IL‐2 and BclX L levels. Here IL‐10 but not transforming growth factor‐β plays a functional role. In summary, CTLA4‐CD80/CD86 interactions on T cells integrate signal strength, based on the dose of Con A, to enhance or inhibit primary mouse CD4 + T cell cycling and survival.