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Anti‐CD25 antibodies affect cytokine synthesis pattern of human dendritic cells and decrease their ability to prime allogeneic CD4 + T cells
Author(s) -
Mnasria K.,
Lagaraine C.,
VelgeRoussel F.,
Oueslati R.,
Lebranchu Y.,
Baron C.
Publication year - 2008
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1007712
Subject(s) - monoclonal antibody , il 2 receptor , cd80 , cd86 , biology , cd40 , immunology , cytokine , stimulation , priming (agriculture) , antibody , microbiology and biotechnology , t cell , immune system , cytotoxic t cell , endocrinology , in vitro , biochemistry , botany , germination
Anti‐CD25 monoclonal antibodies are widely used in clinical transplantation to prevent acute allograft rejection. Although their effects on T lymphocytes have been extensively studied, their impact on human dendritic cells (DC) has never been reported. Furthermore, the role of the IL‐2 in DC functions has not yet been fully elucidated. In this study, we confirm that the stimulation of human monocyte‐derived DC with LPS strongly induced the expression of CD25 and that LPS‐matured DC also expressed the β and γ chain of the IL‐2R. We also showed that adding anti‐CD25 monoclonal antibodies to LPS induced a decrease in IL‐12, IL‐1, TNF‐α, IL‐6, and IFN‐γ production and an increase in IL‐10 synthesis by DC compared with stimulation with LPS alone. Furthermore, we showed that these modifications diminished the T helper priming ability of DC and polarized the alloimmune response toward TH2. In contrast, humanized anti‐CD25 monoclonal antibodies did not affect the up‐regulation of CD86, CD80, CD83, HLADR, or CD40 induced upon LPS stimulation. Taken together, this study discloses some previously unrecognized effects of anti‐CD25 monoclonal antibodies on DC that may contribute to their clinical efficacy. In addition, this study also shed some light on the role of the IL‐2 in human DC activation.