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LTA recognition by bovine γδ T cells involves CD36
Author(s) -
Lubick Kirk,
Jutila Mark A.
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1005616
Subject(s) - ionomycin , biology , cd36 , lipoteichoic acid , cd14 , tlr2 , antigen , t cell , microbiology and biotechnology , monocyte , receptor , immunology , immune system , tlr4 , signal transduction , flow cytometry , biochemistry , intracellular , staphylococcus aureus , bacteria , genetics
CD36 has recently been shown to facilitate monocyte Toll‐like receptor 2 (TLR2) recognition of lipoteichoic acid (LTA), much like CD14 in TLR4 recognition of lipopolysaccharide. We previously found that bovine γδ T cells express CD36 transcripts. Here, we tested whether bovine γδ T cells express CD36 protein and if so, whether it functions in a manner similar to the monocyte molecule. CD36 transcripts and internal and cell surface protein could be detected in resting, sorted γδ T cells. Phorbol 12‐myristate 13‐acetate (PMA)/ionomycin treatment increased CD36 transcript levels (detectable at 4 h) and protein expression (internal and cell surface). Increased surface antigen expression was detectable by 24 h and was maximal at 72 h following PMA/ionomycin stimulation. Anti‐CD36 monoclonal antibody inhibited increased macrophage‐inflammatory protein‐1α gene expression in γδ T cells activated by LTA. In conclusion, γδ T cells express CD36, previously thought to be a myeloid and endothelial cell‐restricted surface antigen, and it contributes to responses by these cells to microbial LTA.