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Molecular characterization of the murine homologue of the DC‐derived protein DC‐SCRIPT
Author(s) -
Triantis Vassilis,
Moulin Veronique,
Looman Maaike W. G.,
Hartgers Franca C.,
Janssen Richard A. J.,
Adema Gosse J.
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1005588
Subject(s) - biology , zinc finger , transcription factor , gene , microbiology and biotechnology , nuclear protein , transcription (linguistics) , synteny , genetics , genome , linguistics , philosophy
Dendritic cell‐specific transcript (DC‐SCRIPT) is a putative DC zinc (Zn) finger‐type transcription factor described recently in humans. Here, we illustrate that DC‐SCRIPT is highly conserved in evolution and report the initial characterization of the murine ortholog of DC‐SCRIPT, which is also preferentially expressed in DC as shown by real‐time quantitative polymerase chain reaction, and its distribution resembles that of its human counterpart. Studies undertaken in human embryonic kidney 293 cells depict its nuclear localization and reveal that the Zn finger domain of the protein is mainly responsible for nuclear import. The human and the mouse genes are located in syntenic chromosomal regions and exhibit a similar genomic organization with numerous common transcription factor‐binding sites in their promoter region, including sites for many factors implicated in haematopoiesis and DC biology, such as Gfi, GATA‐1, Spi‐B, and c‐Rel. Taken together, these data show that DC‐SCRIPT is well‐conserved in evolution and that the mouse homologue is more than 80% homologous to the human protein. Therefore, mouse models can be used to elucidate the function of this novel DC marker.