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Different signaling pathways inhibit DNA methylation activity and up‐regulate IFN‐γ in human lymphocytes
Author(s) -
BonillaHenao Victoria,
Martínez Raquel,
Sobrino Francisco,
Pintado Elizabeth
Publication year - 2005
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1004604
Subject(s) - biology , jurkat cells , dna methylation , methyltransferase , methylation , dna methyltransferase , microbiology and biotechnology , dna , epigenetics , gene , phosphatase , interferon , gene expression , dnmt1 , regulation of gene expression , immune system , enzyme , t cell , biochemistry , genetics
DNA methylation is recognized increasingly for its prominent role in controlling diverse immune processes. In this study, we show that in Jurkat T cells and fresh peripheral lymphocytes, short‐time incubation with protein kinase C activators or phosphatase inhibitors down‐regulate DNA methylation activity in a dose‐dependent manner. This inhibition correlates with the induction of the interferon‐γ (IFN‐γ) gene, which contains several CG sequences in its promoter. The expression of mRNA and protein of the different DNA methyltransferases did not decrease after the treatment. In addition, sulfydryl reagents have a strong inhibitory effect on DNA methylation activity and also induce IFN‐γ gene expression, thus suggesting a link between both effects.