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Signalling via CD70, a member of the TNF family, regulates T cell functions
Author(s) -
García Pilar,
Heredia Agustín Beltrán,
Bellón Teresa,
Carpio Emilio,
Llano Manuel,
Caparrós Esther,
Aparicio Pedro,
LópezBotet Miguel
Publication year - 2004
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1003508
Subject(s) - jurkat cells , biology , microbiology and biotechnology , tumor necrosis factor alpha , transfection , t cell , kinase , cytokine , signal transduction , zap70 , protein kinase b , cell culture , immune system , antigen presenting cell , immunology , genetics
In the present work, we provide data supporting that CD70, a tumor necrosis factor (TNF)‐related molecule, defined as the CD27 ligand (CD27L), may actively regulate T cell functions similarly to other members of the TNF family (i.e., CD40L and CD30L). Cross‐linking CD70 with specific monoclonal antibodies (mAb) stimulated cytotoxicity and cytokine production in human T cell clones. Detection of intracellular‐free calcium mobilization and mitogen‐activated protein kinase phosphorylation upon mAb engagement of CD70 further supported an active signaling role for the TNF‐related molecule. Similar results were obtained in the Jurkat leukaemia T cell line stably transfected with CD70; in that system, induction of Akt phosphorylation was detected, indirectly revealing the involvement of the phosphatidylinositol‐3 kinase pathway. Stimulation of CD70+ Jurkat cells, with a CD70‐specific mAb or with COS‐7 cells transiently transfected with CD27, induced transcriptional activity detectable by different reporter gene expression systems. Altogether, our data point out that a reciprocal communication may be established between CD27+ and CD70+ cells during the immune response.