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Cell surface‐anchored SR‐PSOX/CXC chemokine ligand 16 mediates firm adhesion of CXC chemokine receptor 6‐expressing cells
Author(s) -
Shimaoka Takeshi,
Nakayama Takashi,
Fukumoto Noriko,
Kume Noriaki,
Takahashi Shu,
Yamaguchi Junko,
Minami Manabu,
Hayashida Kazutaka,
Kita Toru,
Ohsumi Jun,
Yoshie Osamu,
Yoehara Shin
Publication year - 2004
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1003465
Subject(s) - cxc chemokine receptors , biology , microbiology and biotechnology , cxcl14 , cxcl16 , chemokine , cxcr3 , chemokine receptor , cxcl9 , xcl2 , ligand (biochemistry) , ccl21 , receptor , cancer research , biochemistry
Direct contacts between dendritic cells (DCs) and T cells or natural killer T (NKT) cells play important roles in primary and secondary immune responses. SR‐PSOX/CXC chemokine ligand 16 (CXCL16), which is selectively expressed on DCs and macrophages, is a scavenger receptor for oxidized low‐density lipoprotein and also the chemokine ligand for a G protein‐coupled receptor CXC chemokine receptor 6 (CXCR6), expressed on activated T cells and NKT cells. SR‐PSOX/CXCL16 is the second transmembrane‐type chemokine with a chemokine domain fused to a mucin‐like stalk, a structure very similar to that of fractalkine (FNK). Here, we demonstrate that SR‐PSOX/CXCL16 functions as a cell adhesion molecule for cells expressing CXCR6 in the same manner that FNK functions as a cell adhesion molecule for cells expressing CX 3 C chemokine receptor 1 (CX 3 CR1) without requiring CX 3 CR1‐mediated signal transduction or integrin activation. The chemokine domain of SR‐PSOX/CXCL16 mediated the adhesion of CXCR6‐expressing cells, which was not impaired by treatment with pertussis toxin, a Gαi protein blocker, which inhibited chemotaxis of CXCR6‐expressing cells induced by SR‐PSOX/CXCL16. Furthermore, the adhesion activity was up‐regulated by treatment of SR‐PSOX/CXCL16‐expressing cells with a metalloprotease inhibitor, which increased surface expression levels of SR‐PSOX/CXCL16. Thus, SR‐PSOX/CXCL16 is a unique molecule that not only attracts T cells and NKT cells toward DCs but also supports their firm adhesion to DCs.