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Streptococcus pyogenes and Lactobacillus rhamnosus differentially induce maturation and production of Th1‐type cytokines and chemokines in human monocyte‐derived dendritic cells
Author(s) -
Veckman Ville,
Miettinen Minja,
Pirhonen Jaana,
Sirén Jukka,
Matikainen Sampsa,
Julkunen Ilkka
Publication year - 2004
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.1003461
Subject(s) - cd80 , biology , lactobacillus rhamnosus , chemokine , cytokine , cd86 , microbiology and biotechnology , streptococcus pyogenes , dendritic cell , immune system , monocyte , interleukin 12 , cd40 , immunology , t cell , cytotoxic t cell , lactobacillus , in vitro , bacteria , staphylococcus aureus , biochemistry , genetics
Dendritic cells (DCs) are the most efficient antigen‐presenting cells and thus, have a major role in regulating host immune responses. In the present study, we have analyzed the ability of Gram‐positie, pathogenic Streptococcus pyogenes and nonpathogenic Lactobacillus rhamnosus to induce the maturation of human monocyte‐derived DCs. Stimulation of DCs with S. pyogenes resulted in strong expression of DC costimulatory molecules CD80, CD83, and CD86 accompanied with a T helper cell type 1 (Th1) cytokine and chemokine response. S. pyogenes also induced interleukin (IL)‐2 and IL‐12 production at mRNA and protein levels. In addition, IL‐23 and IL‐27 subunits p40, p19, p28, and EBI3 were induced at mRNA level. In contrast, L. rhamnosus ‐stimulated DCs showed only moderate expression of costimulatory molecules and produced low levels of cytokines and chemokines. Furthermore, no production of IL‐2 or IL‐12 family cytokines was detected. Bacteria‐induced DC maturation and especially cytokine and chemokine production were reduced when bacteria were heat‐inactivated. Our results show that human monocyte‐derived DCs respond differently to different Gram‐positive bacteria. Although pathogenic S. pyogenes induced a strong Th1‐type response, stimulation with nonpathogenic L. rhamnosus resulted in development of semi‐mature DCs characterized by moderate expression of costimulatory molecules and low cytokine production.