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Transendothelial migration enhances integrin‐dependent human neutrophil chemokinesis
Author(s) -
Gonzalez Anjelica L.,
ElBjeirami Wafa,
West Jennifer L.,
McIntire Larry V.,
Smith C. Wayne
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0906553
Subject(s) - chemokinesis , biology , integrin , chemotaxis , microbiology and biotechnology , neutrophile , neutrophil extracellular traps , immunology , inflammation , receptor , biochemistry
Transendothelial migration of neutrophils induces phenotypic changes that influence the interactions of neutrophils with extravascular tissue components. To assess the influence of transmigration on neutrophil chemokinetic motility, we used polyethylene glycol hydrogels covalently modified with specific peptide sequences relevant to extracellular matrix proteins. We evaluated fMLP‐stimulated human neutrophil motility on peptides Arg‐Gly‐Asp‐Ser (RGDS) and TMKIIPFNRTLIGG (P2), alone and in combination. RGDS is a bioactive sequence found in a number of proteins, and P2 is a membrane‐activated complex‐1 (Mac‐1) ligand located in the γ‐chain of the fibrinogen protein. We evaluated, via video microscopy, cell motility by measuring cell displacement from origin and total accumulated distance traveled and then calculated average velocity. Results indicate that although adhesion and shape change were supported by hydrogels containing RGD alone, motility was not. Mac‐1‐dependent motility was supported on hydrogels containing P2 alone. Motility was enhanced through combined presentation of RGD and P2, engaging Mac‐1, α V β 3 , and β 1 integrins. Naïve neutrophil motility on combined peptide substrates was dependent on Mac‐1, and α 4 β 1 while α 6 β 1 contributed to speed and linear movement. Transmigrated neutrophil motility was dependent on α v β 3 and α 5 β 1 , and α 4 β 1 , α 6 β 1 , and Mac‐1 contributed to speed and linear motion. Together, the data demonstrate that efficient neutrophil migration, dependent on multi‐integrin interaction, is enhanced after transendothelial migration.