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Expression and synthesis of fibroblast growth factor‐9 in human γδ T‐lymphocytes. Response to isopentenyl pyrophosphate and TGF‐β1/IL‐15
Author(s) -
Workalemahu Grefachew,
Foerster Martin,
Kroegel Claus
Publication year - 2004
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0902471
Subject(s) - keratinocyte growth factor , biology , autocrine signalling , fibroblast growth factor , epidermal growth factor , isopentenyl pyrophosphate , transforming growth factor , growth factor , microbiology and biotechnology , receptor , pyrophosphate , biochemistry , enzyme
γδ T‐lymphocytes are believed to play a role in maintaining the normal configuration of epithelial tissue. As little is known about the factors mediating this function, we addressed the question of whether γδ T‐lymphocytes produce fibroblast growth factor (FGF)‐9 as well as two other growth factors associated with epithelial tissue reconstitution. Blood γδ T cells isolated from healthy donors were grown in the presence of isopentenyl pyrophosphate (IPP) or transforming growth factor‐β1 (TGF‐β1)/interleukin‐15 (IL‐15) for 24 h and were assessed for the expression and synthesis of FGF‐9, keratinocyte growth factor (KGF), and epidermal growth factor (EGF). Resting human γδ T cells constitutively expressed KGF and FGF‐9 mRNA but no EGF mRNA. In the presence of IPP, FGF‐9 mRNA expression significantly increased in a dose‐dependent manner, expression of KGF remained unaltered, and EGF mRNA could not be detected. In contrast to IPP, stimulation of the cells with TGF‐β1/IL‐15 did not alter FGF‐9 expression. Moreover, stimulation with anti‐CD3 does not induce FGF‐9 expression but triggers a high signal of interferon‐γ mRNA. Western blot analysis of γδ T cell lysates, prepared 4 days following stimulation with IPP, showed an increase of FGF‐9 protein as compared with control cells. In conclusion, the results demonstrate for the first time that human blood and bronchoalveolar lavage γδ T‐lymphocytes are capable of expressing FGF‐9. The data also provide novel evidence that immunoregulatory cells can synthesize FGF‐9.