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Peritoneal dialysis solutions inhibit the differentiation and maturation of human monocyte‐derived dendritic cells: effect of lactate and glucose‐degradation products
Author(s) -
PuigKröger Amaya,
MuñizPello Oscar,
Selgas Rafael,
Criado Gabriel,
Bajo MAuxiliadora,
SánchezTomero Jose A.,
Alvarez Vicente,
Peso Gloria,
SánchezMateos Paloma,
Holmes Clifford,
Faict Dirk,
LópezCabrera Manuel,
Madrenas Joaquín,
Corbí Angel L.
Publication year - 2003
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0902451
Subject(s) - biology , monocyte , tumor necrosis factor alpha , lipopolysaccharide , microbiology and biotechnology , immune system , peritoneal cavity , immunology , anatomy
Peritoneal dialysis (PD) is a well‐established therapy for end‐stage renal failure, but its efficiency is limited by recurrent peritonitis. As PD solutions impair local inflammatory responses within the peritoneal cavity, we have analyzed their influence on the in vitro maturation of human monocyte‐derived dendritic cells (MDDC). Evaluation of MDDC maturation parameters [expression of adhesion and costimulatory molecules, receptor‐mediated endocytosis, allogeneic T cell activation, production of tumor necrosis factor α, interleukin (IL)‐6 and IL‐12 p70, and nuclear factor (NF)‐κB activation] revealed that currently used PD solutions differentially inhibit the lipopolysaccharide (LPS)‐induced maturation of MDDC, an inhibition that correlated with their ability to impair the LPS‐stimulated NF‐κB activation. Evaluation of PD components revealed that sodium lactate and glucose‐degradation products impaired the acquisition of maturation parameters and NF‐κB activation in a dose‐dependent manner. Moreover, PD solutions impaired monocyte‐MDDC differentiation, inhibiting the acquisition of DC markers such as CD1a and DC‐specific intercellular adhesion molecule‐3 grabbing nonintegrin (CD209). These findings have important implications for the initiation of immune responses under high lactate conditions, such as those occurring within tumor tissues or after macrophage activation.