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The critical role of ocular‐infiltrating macrophages in the development of choroidal neovascularization
Author(s) -
Tsutsumi Chikako,
Sonoda KohHei,
Egashira Kensuke,
Qiao Hong,
Hisatomi Toshio,
Nakao Shintaro,
Ishibashi Minako,
Charo Israel F.,
Sakamoto Taiji,
Murata Toshinori,
Ishibashi Tatsuro
Publication year - 2003
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0902436
Subject(s) - choroidal neovascularization , ccr2 , biology , macrophage , knockout mouse , macular degeneration , tumor necrosis factor alpha , immunology , neovascularization , monocyte , ccl2 , cancer research , pathology , receptor , inflammation , chemokine , angiogenesis , chemokine receptor , medicine , retinal , ophthalmology , genetics , biochemistry , in vitro
Choroidal neovascularization (CNV) is directly related to visual loss in some eye diseases, such as age‐related macular degeneration. Although several human histological studies have suggested the participation of macrophages in CNV formation, the precise mechanisms are still not fully understood. In this study, we elucidated the role of ocular‐infiltrating macrophages in experimental CNV using CCR2 knockout (KO) mice, wild‐type mice, and C57BL/6 (B6) mice. CCR2 is the receptor of monocyte chemoattractant protein‐1, and the number of infiltrating macrophage and the area of CNV were significantly reduced in CCR2 KO mice. Enriched ocular‐infiltrating macrophages from B6 mice actually showed angiogenic ability in a dorsal air sac assay. Moreover, their expression of class II, CD40, B7‐1 and B7‐2 molecules, and the mRNA for potential angiogenic factors, such as vascular endothelial growth factor, basic fibroblast growth factor, and tumor necrosis factor α, was also observed. Collectively, we conclude that ocular‐infiltrating macrophages play an important role in CNV generation.