Premium
The dendritic cell‐derived protein DC‐STAMP is highly conserved and localizes to the endoplasmic reticulum
Author(s) -
EleveldTrancikova Dagmar,
Triantis Vassilis,
Moulin Veronique,
Looman Maaike W. G.,
Wijers Mietske,
Fransen Jack A. M.,
Lemckert Angelique A. C.,
Havenga Menzo J. E.,
Figdor Carl G.,
Janssen Richard A. J.,
Adema Gosse J.
Publication year - 2005
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0804441
Subject(s) - endoplasmic reticulum , biology , microbiology and biotechnology , transmembrane protein , stim1 , green fluorescent protein , postage stamps , fusion protein , gene , biochemistry , recombinant dna , receptor , art , art history
Recently, we described the molecular identification of dendritic cell‐specific TrAnsMembrane protein (DC‐STAMP), a multimembrane‐spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC‐STAMP (mDC‐STAMP) as well as the characterization of the DC‐STAMP protein. The results demonstrate that mDC‐STAMP is over 90% homologous to hDC‐STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC‐STAMP expression is enhanced by interleukin‐4 and down‐regulated upon DC maturation. Analysis of differently tagged DC‐STAMP proteins further demonstrates that hDC‐STAMP and mDC‐STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC‐STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC‐STAMP‐green fluorescent protein fusion protein. These data imply that DC‐STAMP may exert its effect in the ER.