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Adherence influences monocyte responsiveness to interleukin‐10
Author(s) -
PetitBertron AnneFrance,
Fitting Catherine,
Cavaillon JeanMarc,
AdibConquy Minou
Publication year - 2003
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0802388
Subject(s) - monocyte , biology , tumor necrosis factor alpha , tlr4 , interleukin , cytokine , lipopolysaccharide , stat protein , peripheral blood mononuclear cell , receptor , interleukin 19 , immunology , microbiology and biotechnology , signal transduction , stat3 , biochemistry , interleukin 5 , in vitro
We studied the effects of adherence on the properties of interleukin (IL)‐10 on monocyte‐enriched peripheral blood mononuclear cells. We found that the decrease of CD11b expression induced by IL‐10 was enhanced by adherence. Toll‐like receptor (TLR)2 and TLR4 mRNA, as well as TLR4 surface expression, were significantly up‐regulated by IL‐10 in adherent cells. The absence of adherence prevented the inhibitory effects of IL‐10 on lipopolysaccharide‐induced tumor necrosis factor (TNF) and granulocyte‐colony stimulating factor production and increased IL‐1β production and soluble TNF receptor II release in IL‐10‐pretreated cells. Similarly, the absence of adherence amplified the enhancement of phagocytosis induced by IL‐10. Tyk2 and signal transducer and activator of transcription 3 (STAT3) phosphorylation and suppressr of cytokine signaling 3 (SOCS3) expression were induced by IL‐10 in both conditions, but a longer activation and/or expression were observed in adherent monocytes. Finally, heme oxygenase‐1, an anti‐inflammatory molecule, was induced by IL‐10 in adherent monocytes, whereas its expression remained low in nonadherent cells. Altogether, these data illustrate that adherence modulates the properties and the anti‐inflammatory effects of IL‐10.